A new strategy for cellulases application in high temperature industrial scenarios with syringic acid assisting

نویسندگان

چکیده

Abstract In the process of bioethanol production, more stable and active cellulase in high temperature condition is required. this study, syringic acid was applied hydrolysis system. At 70°C, Tv EG3 activity increased 201.36%, Ct BglA decreased 72.79% by acid. With assisting, thermostability improved, reduced. Syringic scarcely affected CBH. system with cellulases containing EG3, CBH, BglA, reducing sugar yield improved 28.37% assisting. molecular dynamic simulation system, backbone root‐mean‐square deviation (RMSD) residue fluctuation (RMSF) CBH reduced, while RMSD RMSF increased. The reduction number secondary structures, especially α‐helix, caused structure presence to collapse at temperature. More structures α‐helix make them temperatures. These means can stabilize structure, destabilize summary, study not only provides insight into assisting but also demonstrates promoting mechanism

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ژورنال

عنوان ژورنال: Gcb Bioenergy

سال: 2023

ISSN: ['1757-1693', '1757-1707']

DOI: https://doi.org/10.1111/gcbb.13052